Bioacoustics Research Lab
University of Illinois at Urbana-Champaign | Department of Electrical and Computer Engineering | Department of Bioengineering
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Your search for ultrasound produced 3296 results.

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Title Cell membrane damage by ultrasound at different cell concentrations.
Author Ellwart JW, Brettel H, Kober LO.
Journal Ultrasound Med Biol
Volume
Year 1988
Abstract Cell membrane damage by ultrasound was studied in human nucleated cells in vitro at various concentrations. Suspensions of human blood cells, cells of a human leukemic cell line (Reh) and mixtures of nucleated cells with erythrocytes were exposed to continuous ultrasound of 782 kHz at a SPTA intensity of 15 W/cm2. The surviving nucleated cells without membrane damage were counted on the basis of exclusion of ethidiumbromide using a flow cytometer. At high cell concentrations as present in whole blood, we observed no cell death, whereas below 5 × 107 cells/ml most of the granulocytes, stimulated lymphocytes and Reh cells were damaged. The concentration threshold below which cells were damaged seemed not to depend on the size of the cells, rather on the concentration of particles in the suspension.


Title Cellular attachment as a sensitive indicator of the effects of diagnostic ultrasound exposure on cultured human cells.
Author Siegel E, Goddard J, James AE Jr, Siegel EP.
Journal Radiology
Volume
Year 1979
Abstract Dispersed cultured human cells (T-1 kidney, FL embryonic kidney, ES.amniotic, and JHA amniotic) were seeded into plastic Petri dishes, incubated.for 45 minutes, exposed to a clinical ultrasound source [total power output,.1.76 +/- 0.18 (S.D.) mW] for 0.25-60 minutes, and the medium replaced..Attachment was significantly reduced after only 0.50 minute of exposure. For.all exposures, sensitivity of the JHA amniotic cells was greater than that of the.kidney lines. Like incremental reduction in attachment, differential sensitivity.among cell lines became less pronounced with protracted exposures. Thus.cellular attachment is a sensitive parameter for studying the effects of.diagnostic ultrasound.


Title Cellular changes due to ultrasound at low intensity levels.
Author Hrazdira I, Adler J, Skorpikova J.
Journal Studia Biophys
Volume
Year 1986
Abstract The cellular effects of pulsed ultrasound at diagnostic in the tensity (SATA - 15 W.m (-2)) have been investigated by means of following experimental methods: cell electrophoresis, electrometric titration of cell surface, electron microscopy and transplantation ability estimation. The results have demonstrated a transient increase in electrophoretic mobility of erytrocytes and bone marrow cells as well as an enhancement of cytochrome-c binding capacity of the cell surface after ultrasonic treatment. The transplantation ability of haemopoietic stem cells was stimulated by ultrasonic action while the elctronmicrographs did not provide morphological evidence for cell surface alterations.


Title Cellular inactivation by heat and shear.
Author Dunn F.
Journal Radiat Environ Biophys
Volume
Year 1985
Abstract Inactivation of Chinese hamster V79 cells in vitro by a temperature elevation to 43 ?C and with Couette shear flow was investigated. The shear stresses were chosen to mimic those produced by ultrasound of approximately 3 MHz and 3 W/cm2 within the chambers employed by earlier investigators studying ultrasonic inactivation of cellular processes. The combined shear and thermal stresses produced survival curves exhibiting a summating effect among these two stresses and remarkably similar to the ultrasound/thermal survival curves.


Title Cellular inactivation by ultrasound.
Author Li GC, Hahn GM, Tolmach LJ.
Journal Nature
Volume
Year 1977
Abstract The lethal effect of ultrasound (US) on mammalian cells has received relatively little attention. Understandably, potential genetic aspects of US have been of prime concern to physicians who use US as a diagnostic tool; at the average power densities involved (<<1 W cm(-2)) little, if any cell killing is to be expected. There have been sporadic attempts to use higher intensities ( approximately 1 W cm(-2)) as a treatment modality in cancer therapy, but those experiments seem to have been based on inadequate cellular studies. The effects of US usually were evaluated in terms of morphological criteria rather than on quantitative determination of the loss of viability as measured by colony formation. There are few reports of the effects of US on survival of mammalian cells, and none specifically examine hyperthermic interaction. With the increased interest in hyperthermia for tumour therapy, attention has been directed towards the use of ultrasound to achieve tumour heating. In preliminary experiments in which US was used to heat the EMT6 sarcoma and KHJJ carcinoma in mice, we found a high percentage of tumour cures with short (approximately 30 min) treatments at temperatures (43-44 degrees C) where in vitro results of hyperthermia-induced cell killing would not have led to a prediction of any cures. We therefore initiated an investigation of the effects of US on survival of Chinese hamster cells to see if direct cell killing by US could explain our in vivo results, or, as in the case of radiofrequency (RF) electromagnetic heating, we would be forced to invoke host response(8). In particular, we examined the thermal and non-thermal components of cellular inactivation by US. We report here that there is a definite non-thermal cytotoxic effect of US. Its relative contribution to cell killing is a highly nonlinear function of the temperature of the cellular milieu. The survival curves show clearly that, beyond an initial threshold, small changes in temperature and/or US intensity can give rise to impressive changes in survival values. The threshold nature of the data strongly suggests that by means of overlapping beams, ultrasound energy could be delivered to tumour tissue to achieve massive cell killings while sparing normal tissue outside the tumour volume to a degree far exceeding that of conventional techniques.


Title Cephalic changes in chick embryos under the action of ultrasound.
Author Vazquez CS.
Journal Ann Otol Rhinol Laryngol
Volume
Year 1963
Abstract No abstract available.


Title Change in the immunogenicity of cells and supernatant in response to ultrasound.
Author Buts VA, Skibenko KP.
Journal Biofizika
Volume
Year 1991
Abstract It has been shown that under the effect of ultrasound of low and middle intensity (I = (0.5 divided by 3) W/cm2, frequency 2.mHz) transition of some antigens from the cell surface to the surrounding liquid takes place. It has been also found that.ultrasound-induced immunogenic pattern of the surface antigens is not lower than the cells immunogenicity.


Title Changes in behavior of mosses treated with ultrasound.
Author Dyer HJ.
Journal Proc Sixtyninth Meet Acoust Soc Am - Washington D.C.
Volume
Year 1965
Abstract No abstract available.


Title Changes in blood flow, oxygen uptake and tissue temperatures produced by therapeutic physical agents. 1. Effect of ultrasound.
Author Abramson DI, Burnett C, Bell Y, Tuck S, Rejal H, Fleischer CJ.
Journal Am J Phys Med
Volume
Year 1960
Abstract No abstract available.


Title Changes in cell ultrastructure under direct and indirect action of ultrasound.
Author Hrazdira I.
Journal Proc First World Congr Ultrason Diagn Med SIDUO III - Vienna
Volume
Year 1969
Abstract No abstract available.


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