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BRL Abstracts Database |
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Your search for ultrasound produced 3296 results. Page 272 out of 330
| Title |
Therapeutic arteriogenesis by ultrasound-mediated VEGF165 plasmid gene delivery to chronically ischemic skeletal muscle. |
| Author |
Leong-Poi H, Kuliszewski MA, Lekas M Sibbald M, Teichert-Kuliszewska K, Klibanov AL, Stewart DJ, Lindner JR. |
| Journal |
Circ Res |
| Volume |
|
| Year |
2007 |
| Abstract |
Current methods of gene delivery for therapeutic angiogenesis are invasive, requiring either intraarterial or intramuscular administration. A noninvasive method of gene delivery has been developed using ultrasound-mediated destruction of intravenously administered DNA-bearing carrier microbubbles during their microcirculatory transit. Here we show that chronic ischemia could be markedly improved by ultrasound-mediated destruction of microbubbles bearing vascular endothelial growth factor-165 (VEGF165) plasmid DNA. Using a model of severe chronic hindlimb ischemia in rats, we demonstrated that ultrasound mediated VEGF165/green fluorescent protein (GFP) plasmid delivery resulted in a significant improvement in microvascular blood flow by contrast-enhanced ultrasound, and an increased vessel density by fluorescent microangiography, with minimal changes in control groups. The improvement in tissue perfusion was attributed predominantly to increases in noncapillary blood volume or arteriogenesis, with perfusion peaking at 14 days after delivery, followed by a partial regression of neovascularization at 6 weeks. Transfection was localized predominantly to the vascular endothelium of arterioles in treated ischemic muscle. RT-PCR confirmed the presence of VEGF165/GFP mRNA within treated ischemic muscle, being highest at day 3 postdelivery, and subsequently decreasing, becoming almost undetectable by 6 weeks. We found a modulation of endogenous growth factor expression in VEGF-treated ischemic muscle, consistent with a biologic effect of ultrasound mediated gene delivery. The results of our study demonstrate the utility of ultrasonic destruction of plasmid-bearing microbubbles to induce therapeutic arteriogenesis in the setting of severe chronic ischemia. |
| Title |
Therapeutic cardiac ultrasound. |
| Author |
Meltzer RS, Schwarz KQ, Mottley JG, Everbach EC. |
| Journal |
Am J Cardiol |
| Volume |
|
| Year |
1991 |
| Abstract |
No abstract available. |
| Title |
Therapeutic potential of low-intensity ultrasound (part 1): Thermal and sonomechanical effects. |
| Author |
Feril LB Jr,Tachibana K,Ogawa K,Yamaguchi K,Solano IG,Irie Y. |
| Journal |
J Med Ultrasonics |
| Volume |
|
| Year |
2008 |
| Abstract |
In this first part of the review, we will focus on and discuss various aspects of low-intensity ultrasound (US), with emphasis on mild thermal effects, apoptosis induction, and sonomechanical effects. Mild thermal effects of US have been commonly applied to physical therapy. Though US has clear beneficial effects, the advantage of using US over other heating modalities remains unclear. US has also been used in vivo and clinically in the treatment of wounds and fractures, with promising results. On the biomolecular level, studies have shown that US can induce apoptosis and that certain conditions can provide optimal apoptosis induction. As to potential therapeutic applications, in addition to the thermal and other physical effects, apoptosis induction by US may offer direct and rapid treatment of tumors or cancer tissues. Technological advances and rapidly accelerating research in this field are providing an ever-increasing array of therapeutic options for lowintensity US.
Keywords low-intensity ultrasound - physical therapy - sonomechanical effects |
| Title |
Therapeutic potential of low-intensity ultrasound (part 2): biomolecular effects,sonotransfection, and sonopermeabilization. |
| Author |
Feril LB Jr,Tachibana K,Ikeda-Dantsuji Y,Endo H,Harada Y,Kondo T,Ogawa R. |
| Journal |
J Med Ultrasonics |
| Volume |
|
| Year |
2008 |
| Abstract |
Part one of this review focused on the thermal and mechanical effects of low-intensity ultrasound (US). In this second and final part of the review, we will focus on and discuss various aspects of low-intensity US, with emphasis on the biomolecular effects, US-mediated gene transfection (sonotransfection), and US-mediated permeabilization (sonopermeabilization). Sonotransfection of different cell lines in vitro and target tissues in vivo have been reported. Optimization experiments have been done and different mechanisms investigated. It has also been found that several genes can be up-regulated or down-regulated by sonication. As to the potential therapeutic applications, systemic or local sonotransfection might also be a safe and effective gene therapy method in effecting the cure of local and systemic disorders. Gene regulation of target cells may be utilized in modifying cellular response to a treatment, such as increasing the sensitivity of diseased cells while making normal cells resistant to the side effects of a treatment. Advances in sonodynamic therapy and drug sonopermeabilization also offer an ever-increasing array of therapeutic options for low-intensity US.
Keywords low-intensity ultrasound - biomolecular effects - sonotransfection - sonopermeabilization |
| Title |
Therapeutic ultrasound and the liver in-vivo. |
| Author |
Pearson D. |
| Journal |
Ultrasound Med Biol |
| Volume |
|
| Year |
1987 |
| Abstract |
Letter to the editor. No abstract available. |
| Title |
Therapeutic ultrasound cavitation and its free radical ESR determination. |
| Author |
Feng R, Qian Y, Xu J, Shi Q, Wang S, Li H. |
| Journal |
Chin J Acoust |
| Volume |
|
| Year |
1991 |
| Abstract |
The formation of free radicals OH and H in a naturally air-saturated aqueous solution exposed to therapeutic CW ultrasound at a frequency of 820 kHz has been confirmed by using spin trapping 5, 5-dimethyl-1-pyrroline-1-oxide (DMPO) and electron spin resonance (ESR) technique. It is suggested that these radicals are formed due to the high temperature and pressure produced by the ultrasonic transient cavitation. The transient cavitation threshold is found at 0.537-0.632W/cm2 under a sonication time of 3 minutes. With increasing sound intensity the yield of free radicals OH raises rapidly at the intensity ranging from 1-2 W/cm2, and no longer increase is observed at above 3W/cm2. The sound intensity (I) dependence of the yield of OH (D) can be approximately described by a regression equation: D = 8.1[(I)(exp)(1/2) - [(I)(sub)(C)](exp)(1/2)](exp)(1/2), where I(sub)C = 0.667 W/cm2. Under a fixing sound intensity the yield of OH increases monotonously with the sonication time. |
| Title |
Therapeutic ultrasound for the treatment of glaucoma. |
| Author |
Silverman RH, Vogelsang B, Rondeau MJ, Coleman DJ. |
| Journal |
Am J Ophthalmol |
| Volume |
|
| Year |
1991 |
| Abstract |
A multicenter clinical trial of therapeutic ultrasound for the treatment of glaucoma included 20 centers in the United States in which 1,117 treatments were performed on 880 eyes. The study was limited to patients with refractory glaucoma who had not benefited from conventional medical and surgical techniques. Approximately 782 of 1,117 treatments (70%) showed an initial decrease in intraocular pressure from a pretreatment mean of 38.1 mm Hg to 22 mm Hg or less. By Kaplan-Meier survival analysis, the single treatment success rate (intraocular pressure between 6 and 22 mm Hg) was 48.7% at six months posttreatment. When retreatment was used subsequent to failure, the one-year multitreatment success rate was 79.3%. The most common complications were an immediate posttreatment intraocular pressure increase lasting a few hours and mild iritis. Other complications included scleral thinning in 28 of 1,117 treatments (2.5%) and phthisis bulbi in 12 of 1,117 treatments (1.1%).
|
| Title |
Therapeutic ultrasound in the production of ocular lesions. |
| Author |
Coleman DJ, Lizzi FL, Jakobiec FA. |
| Journal |
Am J Ophthalmol |
| Volume |
|
| Year |
1978 |
| Abstract |
After testing high intensity focused ultrasound on ocular tissues in animals, to obtain damage threshold equations, we used ultrasound for ocular therapy in experimental animals. We developed a therapeutic system for use in conjunction with a low-energy diagnostic ultrasound visualization technique. Our system included a spherical focused transducer operated at a frequency of 9.8 MHz to produce a focal spot 9 cm from the transducer surface. This technique produced controlled ocular tissue damage similar to that produced by laser and xenon are photocoagulation. It can be used to treat any level of tissue, ocular or orbital, and does not require media clarity. Specific tissue absorption properties necessary for laser damage were not.required to produce the desired damage. |
| Title |
Therapeutic ultrasound in the treatment of glaucoma. I. Experimental model. |
| Author |
Coleman DJ, Lizzi FL, Driller J, Rosado AL, Chang S, Iwamoto T, Rosenthal D. |
| Journal |
Ophthalmology |
| Volume |
|
| Year |
1985 |
| Abstract |
Controlled ultrasonic energy was used to treat a series of laboratory animals in which glaucoma had been induced experimentally. Insonification successfully reduced elevated intraocular pressure in the majority (86%) of test animals. Histopathologic review of globes examined at varying time intervals following treatment showed localized thinning of the sclera with intact conjunctiva, allowing filtration and focal disruption of ciliary epithelium. This technique of treating elevated intraocular pressure in a noninvasive manner offers potential for clinical application in humans. |
| Title |
Therapeutic ultrasound in the treatment of glaucoma. II. Clinical applications. |
| Author |
Coleman DJ, Lizzi FL, Driller J, Rosado AL, Burgess SEP, Torpey JH, Smith ME, Silverman RH, Yablonski ME, Chang S, Rondeau MJ. |
| Journal |
Ophthalmology |
| Volume |
|
| Year |
1985 |
| Abstract |
Focused, high-intensity therapeutic ultrasound was used to treat 69 selected patients with uncontrollably elevated intraocular pressure (IOP). This new technique selectively thins scleral collagen, and produces focal damage to the ciliary epithelium. These tissue modifications provide a reduction in IOP pressure to 25 mmHg or less in 83% of patients with a minimum three-month follow-up period. |
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